白介素10(IL10)檢測試劑盒(化學發光免疫分析法)

CLIA Kit for Interleukin 10 (IL10)

CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor

  • 白介素10(IL10)檢測試劑盒(化學發光免疫分析法) 產品包裝(模擬)
  • 白介素10(IL10)檢測試劑盒(化學發光免疫分析法) 產品包裝(模擬)
  • 白介素10(IL10)檢測試劑盒(化學發光免疫分析法) 實驗結果圖
  • SCA056Mu.jpg 標準曲線圖
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

黑龙江36选7 www.bqukf.com 本試劑盒用于檢測白介素10(IL10),經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的白介素10(IL10)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 84-101 94
EDTA plasma(n=5) 78-93 87
heparin plasma(n=5) 86-101 96

精密度

精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內加入適量的白介素10(IL10),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中白介素10(IL10)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 89-105% 93-101% 85-104% 89-103%
EDTA plasma(n=5) 85-98% 89-96% 82-99% 88-96%
heparin plasma(n=5) 95-103% 83-92% 97-104% 88-96%

穩定性

經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100μL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加底物100µL,37°C孵育10分鐘;
8. 讀數。

實驗原理

將白介素10(IL10)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的白介素10(IL10)與連接于固相載體上的抗體結合,然后加入生物素化的白介素10(IL10)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入底物。加入底物后會產生輝光型光信號。發射光強度和樣品中的白介素10(IL10)呈正相關。用化學發光儀測定相對光單位(RLU),計算樣品濃度。

相關產品

編號 適用物種:Mus musculus (Mouse,小鼠) 應用(僅供研究使用,不用于臨床診斷!)
EPA056Mu61 白介素10(IL10)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA056Mu01 白介素10(IL10)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA056Mu02 白介素10(IL10)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA056Mu61 白介素10(IL10)活性蛋白 Cell?culture;?Activity?Assays.
PAA056Mu01 白介素10(IL10)多克隆抗體 WB; IHC; ICC; IP.
PAA056Mu02 白介素10(IL10)多克隆抗體 WB; IHC; ICC; IP.
LAA056Mu71 白介素10(IL10)多克隆抗體(生物素標記) WB; IHC; ICC.
LAA056Mu81 白介素10(IL10)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
MAA056Mu21 白介素10(IL10)單克隆抗體 WB; IHC; ICC; IP.
HEA056Mu 白介素10(IL10)檢測試劑盒(酶聯免疫吸附試驗法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA056Mu 白介素10(IL10)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA056Mu 白介素10(IL10)檢測試劑盒(酶聯免疫吸附試驗法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA056Mu 白介素10(IL10)檢測試劑盒(化學發光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.

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Toxicon The wound healing potential of a pro-angiogenic peptide purified from Indian Russell's viper (Daboia russelii) venom [Pubmed: 31054289]
中國免疫學雜志 TRAF-6 調控TGF-β1-Smad2/Smad3 信號通路在Graves 病免疫發病機制中的作用 []
Investigación Clínica The impact of IL-10 gene polymorphism 1082A/G (rs1800896) on increased IL-10 secretion in patients with chronic kidney disease in the Kurdistan Region of?… []
Food & Function Network pharmacology oriented study reveals inflammatory state-dependent dietary supplement hepatotoxicity responses in normal and diseased rats [Pubmed: 31140472]
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Antibacterial immunomodulatory and antibiofilm triple effect of Salivaricin LHM against Pseudomonas aeruginosa urinary tract infection model [Pubmed: 31136751]
Journal of the Royal Army Medical Corps CD28 deficiency attenuates primary blast-induced renal injury in mice via the PI3K/Akt signalling pathway [Pubmed: 31129646]
Pinealektomize Edilmi? Septik S??anlarda TNF-α, IL-6, IL-10 ve IL-1β'nin Kan Düzeyleri ile Karaci?er ve B?brekte Gen Ekspresyonu Seviyelerindeki?… [Doi: 10.5505/vtd.2019.32815]
international immunopharmacology Adenovirus-mediated down-regulation of miR-21-5p alleviates experimental autoimmune uveoretinitis in mice [Pubmed: 31228813]
Chemosphere Atmospheric H2S triggers immune damage by activating the TLR-7/MyD88/NF-κB pathway and NLRP3 inflammasome in broiler thymus []
Biomedicine & Pharmacotherapy MODULATION OF LIPOPOLYSACCHARIDE-INDUCED CYTOKINES RESPONSE IN MICE BY GRAVIOLA LEAF POWDER []
Free Radical Biology and Medicine Vitamin D protects against oxidative stress, inflammation and hepatorenal damage induced by acute paracetamol toxicity in rat [Pubmed: 31255668]
international journal of molecular medicine Alcohol?induced attenuation of post?traumatic inflammation is not necessarily liver?protective following trauma/hemorrhage [Pubmed: 31257463]
Environmental Toxicology and Pharmacology Protective effect of Vitamin D3 against lead induced hepatotoxicity, oxidative stress, immunosuppressive and calcium homeostasis disorders in rat []
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