5-羥色胺(5-HT)檢測試劑盒(酶聯免疫吸附試驗法)

ELISA Kit for 5-Hydroxytryptamine (5-HT)

5HT; Serotonin; N-Methyl-Gamma

  • 5-羥色胺(5-HT)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
  • 5-羥色胺(5-HT)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
  • 5-羥色胺(5-HT)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
  • CEA808Ge.jpg 標準曲線圖
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

黑龙江36选7 www.bqukf.com 本試劑盒用于檢測5-羥色胺(5-HT),經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的5-羥色胺(5-HT)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 78-91 81
EDTA plasma(n=5) 98-105 102
heparin plasma(n=5) 89-102 95

精密度

精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內加入適量的5-羥色胺(5-HT),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中5-羥色胺(5-HT)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 78-97% 90-102% 78-102% 78-97%
EDTA plasma(n=5) 80-95% 78-89% 92-105% 78-98%
heparin plasma(n=5) 91-104% 78-103% 80-99% 84-95%

穩定性

經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)50µL,
    加入50µL檢測液A(臨用前配制);
    37°C溫育1小時。
3. 洗板3次;
4. 加檢測溶液B100µL,37°C孵育30分鐘;
5. 洗板5次;
6. 加TMB底物90µL,37°C孵育10-20分鐘;
7. 加終止液50µL,立即450nm讀數。

實驗原理

本試劑盒應用競爭抑制酶聯免疫分析法測定標本中待測物質水平。將5-羥色胺(5-HT)單克隆抗體包被微孔板,制成固相載體,往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原(標準品或樣本),待測抗原與生物素標記抗原對特異性抗體進行競爭結合。溫育后經洗滌去掉未結合物,然后加入HRP標記的親和素,經過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。待測標本濃度越高,標記抗原和抗體的結合就越受到抑制,顯色愈淺。顯色的深淺與酶量呈正相關,而與樣品中待測物質含量呈負相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

相關產品

編號 適用物種:Pan-species (General,通用) 應用(僅供研究使用,不用于臨床診斷!)
CPA808Ge11 5-羥色胺(5-HT)牛血清白蛋白偶聯物 Immunogen; SDS-PAGE; WB.
CPA808Ge21 5-羥色胺(5-HT)卵白蛋白偶聯物 Immunogen; SDS-PAGE; WB.
PAA808Ge01 5-羥色胺(5-HT)多克隆抗體 IHC; ICC; IP.
LAA808Ge71 5-羥色胺(5-HT)多克隆抗體(生物素標記) WB; IHC; ICC.
LAA808Ge81 5-羥色胺(5-HT)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
CEA808Ge 5-羥色胺(5-HT)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA808Ge 5-羥色胺(5-HT)檢測試劑盒(酶聯免疫吸附試驗法,快捷型) Enzyme-linked immunosorbent assay for Antigen Detection.
KSA808Ge11 5-羥色胺(5-HT)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
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Neuroscience Serotonin-Mediated Cardiac Analgesia Via Ah-Type Baroreceptor Activation Contributes to Silent Angina and Asymptomatic Infarction [Pubmed: 31153963]
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