紅細胞生成素(EPO)檢測試劑盒(酶聯免疫吸附試驗法)

ELISA Kit for Erythropoietin (EPO)

EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin

  • 紅細胞生成素(EPO)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
  • 紅細胞生成素(EPO)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
  • 紅細胞生成素(EPO)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
  • SEA028Mu.jpg 標準曲線圖
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

黑龙江36选7 www.bqukf.com 本試劑盒用于檢測紅細胞生成素(EPO),經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的紅細胞生成素(EPO)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 84-102 89
EDTA plasma(n=5) 92-101 95
heparin plasma(n=5) 88-105 95

精密度

精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內加入適量的紅細胞生成素(EPO),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中紅細胞生成素(EPO)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 89-97% 91-99% 96-105% 78-94%
EDTA plasma(n=5) 82-102% 92-99% 82-91% 86-97%
heparin plasma(n=5) 99-105% 85-97% 81-101% 86-94%

穩定性

經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數。

實驗原理

將紅細胞生成素(EPO)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的紅細胞生成素(EPO)與連接于固相載體上的抗體結合,然后加入生物素化的紅細胞生成素(EPO)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的紅細胞生成素(EPO)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

相關產品

編號 適用物種:Mus musculus (Mouse,小鼠) 應用(僅供研究使用,不用于臨床診斷!)
RPA028Mu03 紅細胞生成素(EPO)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA028Mu01 紅細胞生成素(EPO)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA028Mu02 紅細胞生成素(EPO)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA028Mu02 紅細胞生成素(EPO)多克隆抗體 WB; IHC; ICC; IP.
PAA028Mu01 紅細胞生成素(EPO)多克隆抗體 WB; IHC; ICC; IP.
SEA028Mu 紅細胞生成素(EPO)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA028Mu 紅細胞生成素(EPO)檢測試劑盒(酶聯免疫吸附試驗法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA028Mu 紅細胞生成素(EPO)檢測試劑盒(化學發光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.

參考文獻

雜志 參考文獻
Endocrinology Prenatal Glucocorticoid Overexposure Causes Permanent Increases in Renal Erythropoietin Expression and Red Blood Cell Mass in the Rat Offspring [Endo: source]
Journal of Ethnopharmacology Inhibitory effect of polysaccharides isolated from Angelica sinensis on hepcidin expression [PubMed: 21333724]
PLOS ONE CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation [PlosOne: Source]
The Journal of Clinical Endocrinology & Metabolism Alterations of Circulating Endothelial Cell and Endothelial Progenitor Cell Counts around the Ovulation [PubMed: 22948762]
Experimental & Molecular Medicine Long-term and stable correction of uremic anemia by intramuscular injection of plasmids containing hypoxia-regulated system of erythropoietin expression [PubMed: PMC3509184]
Biochem Biophys Res Commun. Reevaluation of erythropoietin production by the nephron [Pubmed:24832733]
Nutrition. Carbohydrate and glutamine supplementation modulates the Th1/Th2 balance after exercise performed at a simulated altitude of 4500 m. [Pubmed:2528040]
J Appl Physiol (1985). Decreased plasma soluble erythropoietin receptor in high-altitude excessive erythrocytosis and Chronic Mountain Sickness [Pubmed:25324511]
Expert Rev Proteomics Plasma hepcidin in early-stage breast cancer patients: no relationship with interleukin-6, erythropoietin and erythroferrone [PubMed: 26496240]
Oncotarget KIAA0101 is associated with human renal cell carcinoma proliferation and migration induced by erythropoietin [pubmed:26575329]
international journal of molecular sciences Expression of Iron-Related Proteins Differentiate Non-Cancerous and Cancerous Breast Tumors. [pubmed:28216608]
Veterinary Clinical Pathology Occult gastrointestinal bleeding is a common finding in dogs with chronic kidney disease [pubmed:28186645]
IOS Press Content Library An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … [articles:journal-of-alzheimers-disease]
Oxidative Medicine and Cellular Longevity Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation [pubmed:29147463]
PLoS?Neglected?Tropical?Diseases Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosis [pubmed:28489854]
Neurochemistry International Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on duration of anoxia [S0197018617304783]
Pesquisa Veterinária Brasileira Relation between anaemia and bone marrow features and serum erythropoietin in dogs with chronic kidney disease [10.1590/s0100-736x2017000600011?]
Medical Journal Armed Forces India Study of acute hypoxia markers in healthy subjects: Utility in post-crash investigation [10.1016/j.mjafi.2017.04.003]
Neurochemistry International Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on?… [Pubmed:29180303]
Clinical and Experimental?Medicine Increased NGAL level associated with iron store in chronic kidney disease with anemia [Pubmed:29909502]
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Bulletin?of Experimental?Biology?and?Medicine Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in?… [Pubmed: 30353332]
FASEB?JOURNAL High-altitude hypoxia decreases plasma erythropoietin soluble receptor (sEpoR) concentration in humans []
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博士論文 PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE []
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